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On the Trail of the Elusive X-Factor: A Sixty-Two-Year-Old Mystery Finally Solved PDF Print E-mail
Written by Chris Masterjohn   
Thursday, 14 February 2008 02:16

Contents

Article Summary

In 1945, Dr. Weston Price described "a new vitamin-like activator" that played an influential role in the utilization of minerals, protection from tooth decay, growth and development, reproduction, protection against heart disease and the function of the brain.

Using a chemical test, he determined that this compound—which he called Activator X—occurred in the butterfat, organs and fat of animals consuming rapidly growing green grass, and also in certain sea foods such as fish eggs.

Dr. Price died before research by Russian scientists became known in the West. These scientists used the same chemical test to measure a compound similar to vitamin K.

Vitamin K2 is produced by animal tissues, including the mammary glands, from vitamin K1, which occurs in rapidly growing green plants.

A growing body of published research confirms Dr. Price's discoveries, namely that vitamin K2 is important for the utilization of minerals, protects against tooth decay, supports growth and development, is involved in normal reproduction, protects against calcification of the arteries leading to heart disease, and is a major component of the brain.

Vitamin K2 works synergistically with the two other "fat-soluble activators" that Price studied, vitamins A and D. Vitamins A and D signal to the cells to produce certain proteins and vitamin K then activates these proteins.

Vitamin K2 plays a crucial role in the development of the facial bones, and its presence in the diets of nonindustrialized peoples explains the wide facial structure and freedom from dental deformities that Weston Price observed.

Main Article (On the Trail of the Elusive X-Factor)

Read in: Czech

In 1945, Weston Price published a second edition of his pioneering work Nutrition and Physical Degeneration, to which he added a new chapter entitled, "A New Vitamin-Like Activator."1 In it, he presented evidence of a theretofore unrecognized fat-soluble substance that played a fundamental role in the utilization of minerals and whose absence from modern nutrition was responsible for the proliferation of dental caries and other degenerative diseases. Although Price quantified the relative amount of this substance in thousands of samples of dairy products sent to him from around the world, he never determined its precise chemical identity. For want of a better means of identification, he referred to it as "Activator X," also sometimes referred to as the "Price Factor."

Price found the highest concentrations of this nutrient in "the milk of several species, varying with the nutrition of the animal" and found the combination of cod liver oil and high-Activator X butter to be superior to that of cod liver oil alone. In the many butter samples he tested, Activator X was only present when the animals were eating rapidly growing green grass. In most regions, this occurred in the spring and early fall.

A Sixty-Year Mystery

For over sixty years, all attempts to identify this elusive "X" factor have failed. In the 1940s, Dr. Royal Lee, founder of the whole food supplement company Standard Process, suggested that activator X was the essential fatty acids.2 In 1980, Dr. Jeffrey Bland suggested more specifically that it was the elongated omega-3 essential fatty acid called EPA.3 Although these fatty acids exert some effects on calcium metabolism,4 neither the distribution of these unsaturated fatty acids in foods nor their chemical behavior corresponds to that of Activator X. Cod liver oil is much richer than butter in essential fatty acids including EPA, and the oils of plant seeds are even richer in these fats, but Price found little, if any, Activator X in these foods. Moreover, Price tested for Activator X by quantifying the ability of a food to oxidize iodide to iodine; essential fatty acids, however, do not possess this chemical ability.

In 1982, one author wrote to the Price-Pottenger Nutrition Foundation that after pursuing a number of false leads while attempting to identify the X factor, he had concluded that the "peculiar behavior" observed in Price's chemical test might be due to a "special kind of oxygen-containing heterocyclic ring," and suggested a compound called 6-methoxybenzoxazolinone (MBOA) as a likely candidate.5 Although researchers first identified MBOA as an antifungal agent found in corn,6 later studies showed that it was found in many other plant foods and acted as a reproductive stimulant in some animals by mimicking the hormone melatonin.7 Although it is present in young, rapidly growing grass, no research has ever established MBOA as an essential nutrient, attributed to it any of the physiological roles of Activator X, or demonstrated its presence in the foods that Price considered to be the richest sources of this nutrient. MBOA, then, was just another false lead; we will soon see, however, that the writer's observations about the chemical nature of Activator X were largely correct.

Vitamin K: Three Discoveries Converge

The test that Price used for Activator X, called iodometric determination, was traditionally regarded within the English language literature as a test for peroxides (carbon-containing molecules that have been damaged by oxygen).8,9 Since peroxides do not have any activity as vitamins, the relationship between the test and any nutritional substance remained a mystery. Although researchers publishing in other languages were using the test to detect a class of chemicals called quinones at least as far back as 1910,10 it was not until 1972 that Danish researchers published a paper in the British Journal of Nutrition showing that the test could be used to detect biological quinones such as K vitamins in animal tissues.12

K vitamins (Figure 1) possess oxygen-containing ring structures that are capable of oxidizing iodide to iodine and would therefore be detected by Price's Activator X test. The K vitamins are likely to go down in history as the most misunderstood group of vitamins of the twentieth century. In many ways, however, modern researchers are now rediscovering properties of these vitamins that Price had discovered over sixty years ago. It has now become clear that both Activator X and its precursor in rapidly growing grass are both members of this group.

There are two natural forms of vitamin K: vitamin K1 and vitamin K2. Vitamin K1, also called phylloquinone, is found in the green tissues of plants, tightly embedded within the membrane of the photosynthesizing organelle called the chloroplast. As the chlorophyll within this organelle absorbs energy from sunlight, it releases high-energy electrons; vitamin K1 forms a bridge between chlorophyll and several iron-sulfur centers across which these electrons travel, releasing their energy so that the cell can ultimately use it to synthesize glucose.13

When animals consume vitamin K1, their tissues convert part of it into vitamin K2,14 which fulfills a host of physiological functions in the animal that we are only now beginning to understand. The ability to make this conversion varies widely not only between species14 but even between strains of laboratory rats,15,16 and has not been determined in humans. The mammary glands appear to be especially efficient at making this conversion, presumably because vitamin K2 is essential for the growing infant.17 Vitamin K2 is also produced by lactic acid bacteria,18 although bacteria produce forms of the vitamin that are chemically different from those that animals produce, and researchers have not yet established the differences in biological activity between these forms.

Although both K vitamins were discovered and characterized over the course of the 1930s, two fundamental misunderstandings about these vitamins persisted for over sixty years: the medical and nutritional communities considered blood clotting to be their only role in the body, and considered vitamins K1 and K2 to simply be different forms of the same vitamin. The first vitamin K-dependent protein relating to skeletal metabolism was not discovered until 1978. It was not until 1997, nearly twenty years later, that the recognition that vitamin K was "not just for clotting anymore" broke out of the confines of the fundamental vitamin K research community.19

Since the amount of vitamin K1 in typical diets is ten times greater than that of vitamin K2,20 researchers have tended to dismiss the contribution of K2 to nutritional status as insignificant. Yet over the last few years, a growing body of research is demonstrating that these two substances are not simply different forms of the same vitamin, but are better seen as two different vitamins: whereas K1 is preferentially used by the liver to activate blood clotting proteins, K2 is preferentially used by the other tissues to place calcium where it belongs, in the bones and teeth, and keep it out of where it does not belong, in the soft tissues.21 Acknowledging this research, the United States Department of Agriculture, in conjunction with researchers from Tufts University, finally determined the vitamin K2 contents of foods in the U.S. diet for the first time in 2006.22

Perfect Correspondence

Because vitamin K1 is directly associated with both chlorophyll and beta-carotene within a single protein complex and plays a direct role in photosynthesis,13 the richness of the green color of grass, its rate of growth, and its brix rating (which measures the density of organic material produced by the plant) all directly indicate its concentration of vitamin K1. Animals grazing on grass will accumulate vitamin K2 in their tissues in direct proportion to the amount of vitamin K1 in their diet. The beta-carotene associated with vitamin K1 will also impart a yellow or orange color to butterfat; the richness of this color therefore indirectly indicates the amount of both vitamins K1 and K2 in the butter. Not only are the K vitamins detected by the Activator X test and distributed in the food supply precisely as Price suggested, but, as shown in Figure 2, the physiological actions that Price attributed to Activator X correspond perfectly to those of vitamin K2. It is therefore clear that the precursor to Activator X found in rapidly growing, green grass is none other than vitamin K1, while Activator X itself is none other than vitamin K2.

Ironically, Price discovered the roles of vitamin K2 in calcium metabolism, the nervous system and the cardiovascular system more than sixty years before the vitamin K research community began elucidating these roles itself, while vitamin K researchers discovered the chemical structure of activator X several years before Price even proposed its existence. Had Price been aware that his chemical test had been used for decades outside of the English language scientific community to detect quinones, a class to which the K vitamins belong, the two independent discoveries of this one vitamin may have converged sooner.

Instead, English-speaking researchers continued for decades to labor under the illusion that the iodometric method detected only peroxides; by the time this illusion was corrected, better methods for detecting peroxides had already been developed, Activator X had been forgotten, and the opportunity to make the connection between these three discoveries was lost. The twenty-first century, however, is already making radical revisions to our understanding of the K vitamins, which now make it clearer than ever that Activator X and vitamin K2 are one and the same.

Synergy with Vitamins A and D

Price showed Activator X to exhibit dramatic synergy with vitamins A and D. Chickens voluntarily consumed more butter and died more slowly on a deficiency diet when the butter was high in both vitamin A and Activator X than when it was high in vitamin A alone. Cod liver oil, which is high in both vitamins A and D, partially corrected growth retardation and weak legs in turkeys fed a deficiency diet, but the combination of cod liver oil and high-Activator X butter was twice as effective. Likewise, Price found that the combination of cod liver oil and a high-Activator X butter oil concentrate was more effective than cod liver oil alone in treating his patients for dental caries and other signs of physical degeneration.

Vitamin K2 is the substance that makes the vitamin A- and vitamin D-dependent proteins come to life. While vitamins A and D act as signaling molecules, telling cells to make certain proteins, vitamin K2 activates these proteins by conferring upon them the physical ability to bind calcium. In some cases these proteins directly coordinate the movement or organization of calcium themselves; in other cases the calcium acts as a glue to hold the protein in a certain shape.33 In all such cases, the proteins are only functional once they have been activated by vitamin K.

Osteocalcin, for example, is a protein responsible for organizing the deposition of calcium and phosphorus salts in bones and teeth. Cells only produce this protein in the presence of both vitamins A and D;34 it will only accumulate in the extracellular matrix and facilitate the deposition of calcium salts, however, once it has been activated by vitamin K2.35 Vitamins A and D regulate the expression of matrix Gla protein (MGP),36,37 which is responsible for mineralizing bone and protecting the arteries from calcification; like osteocalcin, however, MGP can only fulfill its function once it has been activated by vitamin K2.33 While vitamins A and D contribute to growth by stimulating growth factors and promoting the absorption of minerals, vitamin K2 makes its own essential contribution to growth by preventing the premature calcification of the cartilaginous growth zones of bones.38

Vitamin K2 may also be required for the safety of vitamin D. The anorexia, lethargy, growth retardation, bone resorption, and soft tissue calcification that animals fed toxic doses of vitamin D exhibit bear a striking resemblance to the symptoms of deficiencies in vitamin K or vitamin K-dependent proteins. Warfarin, which inhibits the recycling of vitamin K, enhances vitamin D toxicity and exerts a similar type of toxicity itself. Similarly, the same compounds that inhibit the toxicity of Warfarin also inhibit the toxicity of vitamin D. I have therefore hypothesized elsewhere that vitamin D toxicity is actually a relative deficiency of vitamin K2.39 The synergy with which vitamin K2 interacts with vitamins A and D is exactly the type of synergy that Price attributed to Activator X.

Vitamin K2 and Dental Health

Weston Price was primarily interested in Activator X because of its ability to control dental caries. By studying the remains of human skeletons from past eras, he estimated that there had been more dental caries in the preceding hundred years than there had been in any previous thousand-year period and suggested that Activator X was a key substance that people of the past obtained but that modern nutrition did not adequately provide. Price used the combination of high-vitamin cod liver oil and high-Activator X butter oil as the cornerstone of his protocol for reversing dental caries. This protocol not only stopped the progression of tooth decay, but completely reversed it without the need for oral surgery by causing the dentin to grow and remineralize, sealing what were once active caries with a glassy finish. One 14-year-old girl completely healed 42 open cavities in 24 teeth by taking capsules of the high-vitamin cod liver oil and Activator X concentrate three times a day for seven months.

Activator X also influences the composition of saliva. Price found that if he collected the saliva of individuals immune to dental caries and shook it with powdered bone or tooth meal, phosphorus would move from the saliva to the powder; by contrast, if he conducted the same procedure with the saliva of individuals susceptible to dental caries, the phosphorus would move in the opposite direction from the powder to the saliva. Administration of the Activator X concentrate to his patients consistently changed the chemical behavior of their saliva from phosphorus-accepting to phosphorus-donating. The Activator X concentrate also reduced the bacterial count of their saliva. In a group of six patients, administration of the concentrate reduced the Lactobacillus acidophilus count from 323,000 to 15,000. In one individual, the combination of cod liver oil and Activator X concentrate reduced the L. acidophilus count from 680,000 to 0.

In the 1940s, researchers showed that menadione and related compounds inhibited the bacterial production of acids in isolated saliva.47 Menadione itself is a toxic synthetic analogue of vitamin K, but animal tissues are able to convert a portion of it to vitamin K2. The ability of vitamin K-related compounds to inhibit acid production in isolated saliva had no relationship to their vitamin activity, and the most effective of these compounds had practically no vitamin activity at all.48 Researchers unfortunately assumed that because vitamin K did not have a unique role in inhibiting acid formation in saliva within a test tube that it had no nutritional role in preventing tooth decay within living beings.

In 1945, American researchers conducted a double-blind, placebo-controlled trial of menadione-laced chewing gum and showed it to reduce the incidence of new cavities and cause a dramatic drop in the L. acidophilus count of saliva.49 The next year, the Army Medical Department attempted to repeat these results but failed, and research on vitamin K and dental health in the United States was subsequently abandoned.50 The authors of the original study assumed that the menadione exerted its effect simply as a topical anti-bacterial agent, even though it was highly unlikely to sustain a sufficient concentration in the saliva to exert this effect. Ten years later, German researchers showed that injecting menadione into the abdominal cavities of hamsters more effectively prevented tooth decay than feeding it orally.51 Although they could not rule out the possibility that some of this menadione was secreted into the saliva, their results argued in favor of a nutritional role for the vitamin K2 that would have been produced from it. Despite this finding, to this day no one has investigated the role of natural K vitamins in the prevention of dental caries.

Nevertheless, our continually expanding understanding of the physiology of both K vitamins and teeth now makes it clear that vitamin K2 plays an essential role in dental health. Of all organs in the body, vitamin K2 exists in the second highest concentration in the salivary glands (the highest concentration is found in the pancreas). Even when rats are fed only K1, nearly all of the vitamin K in their salivary glands exists as K2.15 Both vitamin K52 and vitamin K-dependent proteins53 are secreted into the saliva, although their function is unknown.

We now know that the growth and mineralization of the dentin that Price observed in response to the combination of cod liver oil and Activator X concentrate would primarily require three essential factors: vitamins A, D, and K2. There are three calcified tissues of the teeth: the cementum forms the roots, the enamel forms the surface, and the dentin forms the support structure beneath it. Cells called odontoblasts lining the surface of the pulp just beneath the dentin continually produce new dentin material. If a cavity invades the dentin and reaches these cells they can die. The pulp tissue, however, contains stem cells that can differentiate into new odontoblasts that could regenerate the lost dentin if the right conditions were present.54

Dentin is unique among the tissues of the teeth for its expression of osteocalcin, a vitamin K-dependent protein better known for its role in organizing the deposition of calcium and phosphorus salts in bone. In the infant rat, whose teeth grow very rapidly, dentin manufactures much more osteocalcin than bone does, suggesting that osteocalcin plays an important role in the growth of new dentin. Matrix Gla protein (MGP), which is required for the mineralization of bone, is also expressed in dentin.55 Vitamins A and D signal odontoblasts to produce osteocalcin,56,57 and probably regulate their expression of MGP as well. Only after vitamin K2 activates these proteins' ability to bind calcium, however, can they lay down the mineral-rich matrix of dentin. The remarkable synergy between these three vitamins exactly mirrors the process Price observed.

Vitamin K2 and Bone Health

Price also believed that Activator X played an important role in bone health. Butter oil concentrate cured rickets and increased serum levels of calcium and phosphorus in rats consuming a mineral-deficient diet. In a four-year-old boy who suffered from rampant tooth decay, seizures and a tendency to fracture, the combination of a large helping of this concentrate and a meal of whole wheat and whole milk rapidly resolved each of these symptoms.

Although the small amount of vitamin D in the butter oil was probably sufficient to cure rickets and the combination of vitamins A and D most likely produced the rise in serum calcium and phosphorus,58 vitamin K2 has a definite role in bone health. There are at least two vitamin K-dependent proteins that fulfill important functions in skeletal metabolism: matrix Gla protein (MGP) and osteocalcin.

In 1997, researchers from the University of Texas and the University of Montreal developed mice that lacked the gene that codes for MGP. These mice appeared normal for the first two weeks of their lives, after which they developed faster heart beats, stopped growing and died within two months with the rupture of their heavily calcified aortas. The disorganization of their cartilage cells not only produced short stature, but also produced osteopenia and spontaneous fractures.38

The bones of mice that lack the osteocalcin gene mineralize just as well as those of mice that do not lack the gene, but the mineral deposits are organized differently. This could mean that osteocalcin is important to the functional quality of bone and the ability to regulate its shape.59 Isolated human osteoblasts, the cells that lay down the calcified matrix of bone, secrete osteocalcin in response to vitamins A and D.34 The protein-rich matrix surrounding these cells will only accumulate this osteocalcin, however, if it is activated by vitamin K2. Calcification of the extracellular matrix occurs in parallel with the accumulation of osteocalcin, but it is not clear whether this protein plays a direct role in laying down the calcium salts or if its accumulation simply reflects the higher amount of vitamin K2 that is available to activate other proteins involved more directly in mineralization such as MGP.35

When there is an insufficient amount of vitamin K to keep up with the production of vitamin K-dependent proteins, many of these proteins are secreted into the blood in an inactive form. Circulating cells then take up these useless proteins and destroy them.40 By drawing a person's blood and testing the percentages of circulating osteocalcin that are active and inactive, we can determine whether that person's bone cells have enough vitamin K to meet their needs. People with the highest percentages of inactive osteocalcin are at a more than five-fold increased risk of hip fracture,60 confirming the value of the test.

By using this test, we can also show that vitamin K2 is the preferred K vitamin of the bones. It takes one milligram per day of a highly absorbable pharmacological preparation of vitamin K1 to maximally activate osteocalcin in human subjects;28 it appears, however, that humans are not capable of absorbing much more than one fifth this amount from whole foods.24 By contrast, large amounts of vitamin K2 are readily absorbed from foods.26 Even when using highly absorbable forms of these vitamins, vitamin K2 is much more effective. Researchers from the University of Maastricht in the Netherlands recently showed that over the course of 40 days, vitamin K2 was three times more effective than vitamin K1 at raising the percentage of activated osteocalcin. Moreover, the effect of vitamin K1 reached a plateau after just three days, whereas the effect of vitamin K2 increased throughout the entire study. Had it lasted longer, the study may have shown an even greater superiority of vitamin K2.32

We can therefore regard the percentage of inactive osteocalcin primarily as a marker for vitamin K2 status. In the healthy adult population, one hundred percent of the vitamin K-dependent blood coagulants produced by the liver are in their active form. By contrast, in this same population between ten and thirty percent of circulating osteocalcin is in its inactive form. Researchers rarely encounter individuals whose osteocalcin is fully activated.31 This suggests that vitamin K2 deficiency is universal, and that variation in K2 status within the population simply reflects varying degrees of deficiency.

Vitamin K1 supplements produce modest decreases in bone loss in the elderly. A number of Japanese trials, on the other hand, have shown that vitamin K2 completely reverses bone loss and in some cases even increases bone mass in populations with osteoporosis.31 The pooled results of seven Japanese trials show that vitamin K2 supplementation produces a 60 percent reduction in vertebral fractures and an 80 percent reduction in hip and other non-vertebral fractures.61 These studies used extremely high amounts of vitamin K2 and did not observe any adverse effects over the course of several years. Since they used such high doses of K2, however, and no studies have tested lower doses, they do not constitute definitive proof that the vitamin activity rather than some drug-like action unique to the high dose produced such dramatic results. The balance of the evidence, however, suggests that vitamin K2 is essential to skeletal health and that it is a key substance that modern diets do not adequately provide.

Vitamin K2 and Heart Disease

Price analyzed more than 20,000 samples of dairy products sent to him every two to four weeks from various districts of the United States, Canada, Australia, Brazil and New Zealand. Dividing the total area into many districts, each producing dairy products with different patterns of seasonal fluctuation in vitamin A and Activator X content, he found an inverse relationship in each district between the vitamin content of butterfat and the mortality from pneumonia and heart disease.

The role of vitamin A in the immune system is well established. We do not currently know, however, whether vitamin K2 plays an important role in the immune system. Nevertheless, lymph glands and bone marrow accumulate large amounts of it62 and a vitamin K-dependent protein called gas6 plays a role in phagocytosis,33 a process wherein immune cells destroy and consume foreign cells or the body's own cells when they are infected or no longer needed. It is therefore possible that K vitamins could play an important role in protecting against infectious diseases such as pneumonia.

Vitamin K2's ability to protect us from heart disease is much more clearly established. Research is in fact rapidly redefining heart disease largely as a deficiency of this vitamin. While it is most clearly established that vitamin K2 deficiency causes calcification of the cardiovascular system, vitamin K2 appears to protect against the inflammation and accumulation of lipids and white blood cells that characterize atherosclerosis as well.

Cardiovascular calcification can begin as early as the second decade of life, and is nearly ubiquitous in the population by the age of 65.33 There are primarily two types: calcification of the heart valves and tunica media constitutes one type, while calcification of the tunica intima constitutes the second. The tunica media is the middle layer of the artery; it contains elastic fibers that allow the artery to stretch and accommodate varying degrees of pressure. The elastic fibers of the tunica media and the valves of the heart calcify during diabetes, kidney disease and aging. The tunica intima is the innermost layer of the artery and is the site where atherosclerosis develops. In atherosclerosis, calcified deposits rich in lipids and white blood cells accumulate on the debris left behind by the blood vessel's smooth muscle cells once they have died.63

In healthy arteries, the vitamin K-dependent matrix Gla protein (MGP) congregates around the elastic fibers of the tunica media and guards them against the formation of crystals by the calcium that circulates in the blood. The inactive form of MGP, which cells produce when they do not have sufficient K vitamins to meet their needs, does not exist in healthy arteries. In early atherosclerosis, by contrast, most MGP exists in its inactive form and associates with calcified structures containing lipids, white blood cells, and the remnants of dead smooth muscle cells. Inactive MGP also accumulates within the calcified deposits of the medial sclerosis that occurs during diabetes, kidney disease and aging. Although blood tests for the percentage of inactive and active MGP are not available, patients with severe calcifications have high percentages of inactive osteocalcin, indicating a general deficiency of vitamin K2.63

Two other vitamin K-dependent proteins are likely to play a role in the development of atherosclerosis: gas6 and protein S. Gas6 promotes the survival of the smooth muscle cells that line the intima and the rapid clearance of those that die. The rapid clearance of these dead cells may be important for preventing the accumulation of the calcified lipids and white blood cells that gather around them. Protein S guides the immune system to clear away this debris from the intima gently rather than mounting a dangerous inflammatory attack against it.33 As these observations all predict, experimental and epidemiological evidence both show that vitamin K2 is a powerful inhibitor of cardiovascular disease.

Mice that lack the gene for MGP develop extensive calcification of the aorta, aortic valves and arteries soon after birth and bleed to death within two months when their heavily calcified aortas rupture.38 Warfarin, which inhibits the recycling of K vitamins40 and the conversion of K1 to K2,64 causes calcification of the tunica media in rats within two weeks,21 increases arterial stiffness, decreases the ability of the artery to accommodate moderately high levels of blood pressure, and causes the death of the artery's smooth muscle cells.65 Marcoumar, a similar drug, doubles the degree of aortic valve calcification in humans over the course of one to three years.42

Large amounts of vitamin K2 completely inhibit the ability of Warfarin to cause arterial calcification in rats. Vitamin K1, by contrast, has no inhibitory effect at all.21 Researchers from the University of Maastricht recently showed that both K vitamins can reverse calcification that has already occurred in Wistar Kyoto rats.65 The K vitamins also reduced the number of dead smooth muscle cells after Warfarin treatment, showing that vitamin K-dependent proteins not only promote cell survival but also facilitate the safe clearance of cells that have died. Although both K vitamins were effective, these rats convert vitamin K1 to vitamin K2 with great efficiency. In the absence of Warfarin, two-thirds of the vitamin K in the blood vessels of the rats that consumed K1 alone existed as K2. In the presence of Warfarin, however, which inhibits the conversion, none of the vitamin K in these blood vessels existed as K2. Apparently, vitamin K1 is effective after but not during Warfarin treatment because it can only protect against arterial calcification insofar as it is converted to vitamin K2.

In the Nurses' Health Study, the risk of heart disease was a modest 16 percent lower for those consuming more than 110 micrograms per day of vitamin K1, but there was no benefit from consuming any more than this.66 This small amount is equivalent to consuming only three servings of kale per month. The Health Professionals Follow-Up Study generated a similar finding in men, although it lost significance after adjustment for other dietary risk factors.67 It isn't clear whether the slight increase in risk associated with only the lowest intakes reflects the possibility that only very small amounts of vitamin K1 are absorbed, or simply reflects the association between K1 intake and a healthy lifestyle. People who consume more vitamin K1 weigh less, smoke less, eat more fruits, vegetables, fish, folate, vitamin E and fiber,68 and are more likely to use vitamin supplements.67

The inverse association between heart disease and vitamin K2 intake is more straightforward. In The Rotterdam Study, which prospectively followed just over 4,600 men aged 55 or older in the Netherlands, the highest intake of vitamin K2 was associated with a 52 percent lower risk of severe aortic calcification, a 41 percent lower risk of coronary heart disease (CHD), a 51 percent lower risk of CHD mortality, and a 26 percent lower risk of total mortality. Even though the study population consumed ten times more K1 than K2, vitamin K1 had no association with either the degree of aortic calcification or the risk of heart disease.20 The profound effects of variations in such small amounts of dietary K2 emphasize just how powerful this substance is in the prevention of degenerative disease.

Vitamin K2 and the Brain

Price supplied several anecdotes suggesting that Activator X plays an important role in the nervous system. Price administered a daily meal of nutrient-dense whole foods supplemented with high-vitamin cod liver oil and high-Activator X butter oil to the children of impoverished mill workers who suffered from rampant tooth decay. The treatment not only resolved the tooth decay without the need for oral surgery, but resolved chronic fatigue in one boy and by the report of their school teachers produced a marked increase in learning capacity in two others.

Price also administered the butter oil concentrate to a four-year-old who suffered from rampant tooth decay, a fractured leg and seizures. A dessert spoonful of the butter oil served over whole wheat gruel with whole milk once before bed and five times over the course of the following day immediately resolved his seizures. Rapid healing of his fracture and dental caries followed soon after. The fact that these three symptoms appeared together and resolved following the same treatment suggests a common cause for each of them. Sixty years later, modern research is now elucidating the essential role that vitamin K2 plays not only in the dental and skeletal systems, but in the nervous system as well. This strongly suggests it was the key unidentified factor in Price's protocol.

The brain contains one of the highest concentrations of vitamin K2 in the body; only the pancreas, salivary glands, and the cartilaginous tissue of the sternum contain more. When male Wistar rats consume vitamin K1 alone, 98 percent of the vitamin K in their brains exists as K2, demonstrating the overwhelming preference of the nervous system for this form. The K2 contents of these four tissues remain remarkably high on a vitamin K-deficient diet, suggesting either that the vitamin is so essential to their function that they have developed a highly efficient means of preserving it, or that it plays a unique role in these tissues that does not require as high a rate of turnover as is required by the roles it plays in most other tissues.15

An analysis of three autopsies showed that vitamin K2 makes up between 70 and 93 percent of the vitamin K in the human brain.69 It is not clear why humans exhibit greater variation in this percentage than rats, although it could be that we convert K1 less efficiently and are therefore more dependent on dietary K2.

Vitamin K2 supports the enzymes within the brain that produce an important class of lipids called sulfatides. The levels of vitamin K2, vitamin K-dependent proteins and sulfatides in the brain decline with age; the decline of these levels is in turn associated with age-related neurological degeneration.46 Comparisons of human autopsies associate the early stages of Alzheimer's disease with up to 93 percent lower sulfatide levels in the brain.70 Warfarin treatment or dietary vitamin K deficiency causes lack of exploratory behavior and reduced physical activity in rats that is suggestive of fatigue.71 Animals that completely lack the enzymes to make sulfatides and a related class of lipids, cerebrosides, progressively suffer from growth retardation, loss of locomotor activity, weak legs and seizures.72

These observations suggest that deficiencies in vitamin K, especially vitamin K2, could result in fatigue and learning difficulties in humans, and that rare, extreme deficiencies of vitamin K2 in the brain could result in seizures. If this is the case, it would explain why Price observed tooth decay, bone fracture, learning difficulties and seizures to share a common cause and a common solution.

Other Roles of Vitamin K2

Our understanding of the K vitamins is rapidly expanding and we are likely to discover many new roles for them as the twenty-first century progresses.

The highest concentration of vitamin K2 exists in the salivary glands and the pancreas. These organs exhibit an overwhelming preference for K2 over K1 and retain high amounts of the vitamin even when animals consume a vitamin K-deficient diet.15 The high presence of the vitamin in both of these organs suggests a role in activating digestive enzymes, although its apparent role in the regulation of blood sugar could explain its presence in the pancreas.76 The testes of male rats also exhibit a high preference for and retention of vitamin K2,16 and human sperm possess a vitamin K-dependent protein with an unknown function.77 The kidneys likewise accumulate large amounts of vitamin K269 and secrete vitamin K-dependent proteins that inhibit the formation of calcium salts. Patients with kidney stones secrete this protein in its inactive form, which is between four and twenty times less effective than its active form at inhibiting the growth of calcium oxalate crystals, suggesting that vitamin K2 deficiency is a major cause of kidney stones.77

The use of Warfarin during pregnancy produces developmental malformations of the face; as the nasal cartilage calcifies, growth of the nose comes to an early end, resulting in a stubby appearance.78 Vitamin K2 therefore most certainly played a role in the development of beautiful faces with broad features that Price observed among primitive peoples.

A number of cell experiments have shown that vitamin K2 has powerful anti-carcinogenic properties that may make it useful in preventing or treating cancer in humans.79

Researchers have recently discovered a whole new class of vitamin K-dependent proteins called transmembrane Gla (TMG) proteins. Their functions are unknown.33

The K vitamins perform all of their well understood roles in the part of the cell responsible for the modification of proteins. Only a portion of the vitamin K within a cell exists in this area, however. Even more exists in the inner membrane of the mitochondria where the cell produces its energy.45 The greatest concentration exists in the nucleus, which possesses a receptor for vitamin K that may be involved in regulating the expression of genes.44 Vitamin K2 has a greater affinity than vitamin K1 for both the mitochondrial membrane and the nuclear receptor. We presently know virtually nothing about these functions of the K vitamins and the plot will only thicken as the story unfolds.

Vitamin K2 in Foods

Figure 4 shows the distribution of vitamin K2 in selected foods. Precise values for the organ meats that would be richest in K2 are not available. The pancreas and salivary glands would be richest; reproductive organs, brains, cartilage and possibly kidneys would also be very rich; finally, bone would be richer than muscle meat.15,16,69 Analyses of fish eggs, which Price found to be rich in Activator X, are not available.

Commercial butter is only a moderate source of vitamin K2. After analyzing over 20,000 samples of butter sent to him from around the world, however, Price found that the Activator X concentration varied 50-fold. Vitamin K-rich cereal grasses, especially wheat grass, and alfalfa in a lush green state of growth produced the highest amounts of Activator X, but the soil in which the pasture was grown also profoundly influenced the quality of the butter. The concentrations were lowest in the eastern and far western states where the soil had been tilled the longest, and were highest in Deaf Smith County, Texas, where excavations proved the roots of the wheat grass to pass down six feet or more through three feet of top soil into deposits of glacial pebbles cemented together with calcium carbonate. It was this amazingly vitamin-rich butter that had such dramatic curative properties when combined with high-vitamin cod liver oil and nutrient-dense meals of whole milk, whole grains, organ meats, bone broths, fruits and vegetables.

For over 50 years after Price described his discovery of Activator X, the medical and nutritional communities saw vitamin K merely as a requirement for blood clotting. The poor understanding of the functions of the K vitamins within the body and the apparent lack of any relationship between Price's chemical test and the structure of any known vitamin made it impossible to determine the identity of this mysterious substance. We now know, however, that vitamin K2 and Activator X are one and the same. Like Price's X factor, vitamin K2 is synthesized by animal bodies from its precursor in rapidly growing grass. Cereal grasses and alfalfa are rich in this precursor, and these plants accumulate it in direct proportion to their photosynthetic activity. It is critical to the ability of teeth and bones to lay down mineralized tissue, and to the prevention of degenerative diseases of the cardiovascular and nervous systems. It is the key factor that acts in synergy with vitamins A and D: these vitamins command cells to make proteins, but vitamin K brings these proteins to life. It is an "activator," then, in the truest sense of the word, and it is therefore fitting that we knew it for so many decades simply as "Activator X."

Thank you to Michael Eiseike, a health researcher from Hokkaido Japan, for originally bringing the Rotterdam Study to our attention and suggesting that vitamin K2 may be the X Factor of Weston Price; and also to David Wetzel of Green Pasture Products for his input and advice.

Figures

Figure 1: The Structure of K Vitamins and Their Chemical Behavior

Single lines represent single bonds between carbon atoms; double lines represent double bonds between carbon atoms. Hydrogen atoms are attached to most of the carbons but are not shown.

a. abc-vitk1a

Vitamin K1. The side chain extending to the right of the molecule is monounsaturated.

b. abc-vitk1b

Vitamin K2. The nucleus, composed of two ring structures, is the same as that of vitamin K1. The side chain, however, is polyunsaturated rather than monounsaturated.

c. abc-vitk1c

Either K vitamin would be expected to react with hydriodic acid (HI) by absorbing hydrogen atoms and liberating diatomic iodine (I2). The side chain is abbreviated by the letter "R."

d. abc-vitk1d

If the mixture of the vitamin K and hydriodic acid is combined with a starch indictor, the diatomic iodine liberated by the reaction would turn the starch blue.

Figure 2. Corresponding Characteristics of Activator X and Vitamin K2

Activator X

Vitamin K2

Found in the butterfat of mammalian milk, the eggs of fishes, and the organs and fats of animals.

Found in the butterfat of mammalian milk and the organs and fats of animals. Analyses of fish eggs are not available.

Synthesized by animal tissues, including the mammary glands, from a precursor in rapidly growing, green grass. Synthesized by animal tissues, including the mammary glands, from vitamin K1, which is found in association with the chlorophyll of green plants in proportion to their photosynthetic activity.
The content of this vitamin in butterfat is proportional to the richness of its yellow or orange color. Its precursor is directly associated with beta-carotene, which imparts a yellow or orange color to butterfat.
Liberates diatomic iodine from hydriodic acid during chemical testing. Liberates diatomic iodine from hydriodic acid during chemical testing.
Acts synergistically with vitamins A and D. Activates proteins that cells are signaled to produce by vitamins A and D.
Plays an important role in reproduction. Synthesized by the reproductive organs in large amounts from vitamin K1 and preferentially retained by these organs on a vitamin K-deficient diet. Sperm possess a K2-dependent protein of unknown function.
Plays a role in infant growth. Contributes to infant and childhood growth by preventing the premature calcification of the cartilaginous growth zones of bones.
Plays an essential role in mineral utilization and is necessary for the control of dental caries. Activates proteins responsible for the deposition of calcium and phosphorus salts in bones and teeth and the protection of soft tissues from calcification.
Increases mineral content and decreases bacterial count of saliva. Is found in the second highest concentration in the salivary glands, and is present in saliva.
Intake is inversely associated with heart disease. Protects against the calcification and inflammation of blood vessels and the accumulation of atherosclerotic plaque.
Increases learning capacity. The brain contains one of the highest concentrations of vitamin K2, where it is involved in the synthesis of the myelin sheath of nerve cells, which contributes to learning capacity.
Resolved chronic fatigue in one boy. Deficiency induces fatigue in laboratory animals.
Resolved seizures in one boy. Involved in the synthesis of lipids called sulfatides in the brain, an absence of which induces seizures in laboratory animals.

Figure 3. Vitamin K-Dependent Carboxylation

abc-vitk1e

a.) A carbon dioxide molecule
b.) a carboxyl group
c.) Vitamin K-dependent carboxylation

The vitamin K-dependent carboxylase rearranges the chemical bonds within carbon dioxide molecules. Carboxyl groups contain carbon and oxygen atoms and carry a charge of negative one. Calcium carries a charge of positive two. The side chains of the amino acid glutamate normally carry one carboxyl group; the vitamin K-dependent addition of a second carboxyl group gives these side chains a charge of negative two and thus allows them to bind to calcium, which has the equal and opposite charge. This process transforms glutamate into γ-carboxyglutamate, abbreviated Gla. For this reason, many vitamin K-dependent proteins, such as matrix Gla protein (MGP), contain "Gla" in their name.

Figure 4: Vitamin K2 Contents of Selected Foods22, 26

The percentage of vitamin K2 present as MK-4 represents that synthesized by animal tissues, while the remainder represents that synthesized by bacteria during fermentation.

FOOD
VITAMIN K2 (MCG/100G)
Natto
1103.4 (0% MK-4)
Goose Liver Paste
369.0 (100% MK-4)
Hard Cheeses
76.3 (6% MK-4)
Soft Cheeses
56.5 (6.5% MK-4)
Egg Yolk (Netherlands)
32.1 (98% MK-4)
Goose Leg
31.0 (100% MK-4)
Curd Cheeses
24.8 (1.6% MK-4)
Egg Yolk (United States)
15.5 (100% MK-4)
Butter
15.0 (100% MK-4)
Chicken Liver
14.1 (100% MK-4)
Salami
9.0 (100% MK-4)
Chicken Breast
8.9 (100% MK-4)
Chicken Leg
8.5 (100% MK-4)
Ground Beef (Medium Fat)
8.1 (100% MK-4)
Bacon
5.6 (100% MK-4)
Calf Liver
5.0 (100% MK-4)
Sauerkraut
4.8 (8% MK-4)
Whole Milk
1.0 (100% MK-4)
2% Milk
0.5 (100% MK-4)
Salmon
0.5 (100% MK-4)
Mackerel
0.4 (100% MK-4)
Egg White
0.4 (100% MK-4)
Skim Milk
0.0
Fat-Free Meats
0.0

SIDEBARS

The Activator X Test

The chemical test that Price eventually came to use for the quantification of Activator X in foods was originally suggested as an indirect test for vitamin D by Lester Yoder of the Agricultural Experiment Station of Iowa State College in 1926.8 The basic principle of the test, called iodometric determination, was most commonly utilized in the United States for detecting the presence of organic peroxides.9 Since peroxides are capable of oxidizing ionic iodide to diatomic iodine, researchers can detect them by combining the test substance with hydriodic acid and a starch indicator. Hydriodic acid releases iodide ions into a solution. If peroxides are present, they convert these iodide ions to diatomic iodine, which then turns the starch blue or purple.

This is somewhat similar to the amylase test that is used as a demonstration in many high school or college biology classes. In that test, however, preformed iodine is used; in the absence of amylase, the iodine turns the starch blue, while in the presence of amylase, the starch is broken down into sugar and the color change does not occur.

At the time, the only way to test a food for vitamin D was to feed it to rats on a mineral-deficient diet, kill the rats, and analyze the mineral content of their bones. The richer the food was in vitamin D, the more it would stimulate absorption of the small amounts of calcium and phosphorus in the diet and the higher the bone mineral content would be. Yoder suggested, however, that there was a general correlation between the ability of an oil to peroxidize (become rancid) and its vitamin D content, and advocated testing an oil's ability to oxidize iodide as an indirect indicator of its level of vitamin D. Having no other convenient chemical test, Price adopted this as his test for vitamin D.

The test was far from perfect. Yoder found peroxidation in substances with no vitamin D activity such as turpentine, a thirteen-year-old sample of cholesterol, and an aged sample of mineral oil. He further found that irradiating foods to the point at which their vitamin D activity was destroyed actually increased their score on the test.8

As Price used this test on over 20,000 samples of dairy foods sent to him from around the world, he realized that the physiological effects that correlated with a food's ranking were different from those attributable to isolated vitamin D, and began using the term "Activator X" to describe the nutritional substance that the test was measuring. He observed that the vitamin content of these butter samples varied fifty-fold, and that the samples richest in Activator X were the most potent for controlling dental caries. Clearly, Price's test was detecting something besides rancid oils.

While researchers who published in English language journals traditionally used this test to detect peroxides, researchers publishing in Russian and German language journals had been using it to detect the synthetic compound benzoquinone all along.10,11 Benzoquinone belongs to a class of chemicals called quinones that includes biological molecules such as coenzyme Q10 and the K vitamins. These quinones possess oxygen-containing ring structures whose oxygens will steal electrons and hydrogen ions from hydriodic acid and thereby oxidize ionic iodide to diatomic iodine, causing the starch to become a bluish purple color (see Figure 1).

In the 1970s, researchers from Britain and Denmark were debating whether or not healthy rat tissues contained lipid peroxides. The British researchers used the iodometric method to determine peroxide levels and argued that healthy rat tissues did contain peroxides, while the Danish researchers used a different method and argued that they did not. In a 1972 paper published in the British Journal of Nutrition, the Danish researchers demonstrated that the iodometric method was not showing the existence of peroxides in the rat tissues, but rather the existence of coenzyme Q10 and probably other quinones.12

Price's test, therefore, was not specific to any one particular chemical compound. When used for fresh oils, however, it would be able to detect a number of nutrients that include coenzyme Q10 and the K vitamins. As shown in this article, it is the K vitamins that we should expect to vary in direct proportion to the amount of richly green grass in the diet of the animals, while the physiological effects Price identified with Activator X are specifically attributable to vitamin K2.


Interactions between Vitamins A, D, and K2

SOFT TISSUE CALCIFICATION AND VITAMIN D TOXICITY
(Hypothesis)

Vitamin Karrowright

Vitamin D

arrowleftVitamin A

Fulfills demand for Vitamin K

arrowdown

Exerts Vitamin K sparing effect
May protect by other unknown mechanisms

Increased demand
for Vitamin K

arrowdown

Relative deficiency
of Vitamin K

arrowdown

 

Soft tissue calcification
Bone loss, growth retardation
Nervous system damage

 

BONES & TEETH

Vitamin A

Vitamin D

 

Vitamin A

Vitamin D

arrowdownright

arrowdownleft

arrowdownright

arrowdownleft

Matrix Gla Protein

 

Osteocalcin

arrowdown

arrowleftVitamin Karrowright

arrowdown

Activated Matrix Gla Protein

 

Activated Osteocalcin

arrowdown

arrowdown

Deposition of Minerals

Organization of Minerals

 

GROWTH

Vitamin A

Vitamin D

Vitamin K

arrowdown

arrowdown

arrowdown

Synthesis of Growth Factors and Growth Factor Receptors

Absorption of Minerals

Prevention of the Calcification of Growth Cartilage

arrowdownright

arrowdown

arrowdownleft

OPTIMAL GROWTH & DEVELOPMENT

Strong Bones
Straight Teeth
Good Proportions
Wide Facial Development
Long Straight Nose


Is Vitamin K2 an Essential Nutrient?

Vitamins K1 and K2 are both effective cofactors for the enzyme that activates vitamin K-dependent proteins,23 but the liver preferentially uses vitamin K1 to activate clotting factors while most other tissues preferentially use vitamin K2 to activate the other vitamin K-dependent proteins.21 Although animals can convert vitamin K1 to vitamin K2,14 there are a number of lines of evidence strongly suggesting that humans require preformed K2 in the diet to obtain optimal health.

Humans appear to have a finite ability to absorb vitamin K1 from plant foods. In the United States, where the mean intake of vitamin K1 is less than 150 micrograms per day, blood levels increase with increasing dietary intake until the latter reaches two hundred micrograms per day, after which they plateau. In the Netherlands, where the mean intake of vitamin K1 is much higher (250 micrograms per day), plasma levels of vitamin K1 have no relationship to dietary intake at all.24 These results suggest that humans do not possess the ability to absorb much more than 200 micrograms of vitamin K1 per day from vegetables.

This interpretation is also supported by feeding experiments. Whereas the absorption of vitamin K2 from natto, a fermented soy food, is nearly complete, the absorption of vitamin K1 from servings of green vegetables ranging from two hundred to four hundred grams consumed without added fat is only between five and ten percent. The absorption of similarly sized servings of vegetables with added fat is still only between ten and fifteen percent.25-26 By contrast, smaller servings are absorbed more efficiently. For example, the absorption from a 150-gram serving of spinach is 17 percent and the absorption from a 50-gram serving of spinach is 28 percent.27 These results show that our absorption of the vitamin declines as the amount we consume increases and strengthens the interpretation that we might only be able to absorb about 200 micrograms per day. When study subjects consume a highly absorbable pharmacological preparation of vitamin K1, a dose of 1000 micrograms per day is required to maximize the activation of proteins important to bone metabolism.28 If we can only absorb one-fifth of this amount from vegetables, we cannot support our skeletal system with vitamin K1 regardless of how efficiently we may be able to convert it to vitamin K2.

The ability to convert K1 to K2 varies widely between species and breeds of animals. The German researchers who first reported this conversion found that rats made it poorly compared to birds and that pigeons made it most efficiently.14 Every tissue tested in male Wistar rats is capable of making the conversion,15 whereas the liver, kidneys and heart of male Lewis rats will preferentially accumulate preformed K2, but, unlike the pancreas and testes of these same animals, will not synthesize it from K1.16 The K2 content of human breast milk increases when mothers consume pharmacological preparations of K1, but the K2 content of their blood does not;17 since the conversion takes place in the target tissues rather than the blood, however, we do not know how efficiently other human tissues make this conversion.

Vitamins K1 and K2 share a common ring-structured nucleus but possess different types of side chains. The first step in the conversion of K1 to K2 appears to be the cleavage of its side chain in either the liver or the gastrointestinal tract, yielding a toxic oxidizing agent called menadione; much of this metabolite is detoxified by the liver and excreted in the urine, while the remaining portion can be used to synthesize K2 in tissues.29 After this cleavage takes place, menadione must be transported to its target tissues where cellular enzymes can add a side chain to it, completing the transformation to K2. Because they are transported in different types of lipoproteins, vitamin K1 is primarily sent to the liver, whereas vitamin K2 is primarily sent to the other tissues;30 we know very little, however, about the transport of menadione in the blood. We also know very little about the rate at which our cells are capable of adding side chains to these molecules; presumably, if the supply of menadione exceeds the rate at which the cell can add these side chains, the menadione will exert toxic effects and cause oxidative damage within the cell. Preliminary evidence indicates that doses of 1000 micrograms per day of supplemental K1 may contribute to periodontal disease,31 suggesting that our bodies' resistance to absorbing this much K1 from vegetables may serve an important purpose.

The clearest demonstration that humans require dietary preformed vitamin K2 for optimal health is that epidemiological and intervention studies both show its superiority over K1. Intake of vitamin K2, for example, is inversely associated with heart disease in humans while intake of vitamin K1 is not,20 and vitamin K2 is at least three times more effective than vitamin K1 at activating proteins related to skeletal metabolism.32 This nutritional superiority makes it clear why the primitive groups that Weston Price studied expended so much effort procuring foods rich in vitamin K2 like the organs and fats of animals and the deeply colored orange butter from animals grazing on rich pastures.


The Vitamin K-Dependent Carboxylase

Most known functions of the K vitamins are mediated by the vitamin K-dependent carboxylase. The carboxylase is an enzyme bound to the membrane of the endoplasmic reticulum, a cellular organelle involved in the synthesis and modification of proteins. It uses vitamin K as a cofactor to add carboxyl groups to the side chains of the amino acid glutamate within certain vitamin K-dependent proteins (see Figure 3). This gives them a negative charge, allowing them to bind to calcium, which carries a positive charge.40

Vitamin K-dependent proteins must be carboxylated before they leave the cell or insert themselves into its membrane. They may contain anywhere from three to thirteen glutamate residues (amino acids are called "residues" when they are bound up within proteins) that must be carboxylated; the carboxylase binds to them only once, however, and carboxylates each of these before it releases the protein. On the other hand, vitamin K can only be used for the carboxylation of a single glutamate residue and the carboxylase must release it after each carboxylation and allow it to be recycled and returned. A different enzyme, vitamin K oxidoreductase, recycles the vitamin; this enzyme is the target of the anticoagulant drug Warfarin and its relatives.40 Since Warfarin targets the recycling of vitamin K rather than the vitamin K-dependent coagulation proteins themselves, it not only acts as an anticoagulant, but also causes arterial and aortic valve calcification in both rats21 and humans41,42 and inhibits the mineralization of bone matrix.35

The distribution of the carboxylase among species and among tissues within an organism can help us understand its significance and that of its cofactor, vitamin K. With the exception of some microorganisms that have "stolen" the enzyme by incorporating the genetic material of other species,43 the carboxylase is present only in multicellular animals, underscoring its importance to intercellular communication. In the growing embryo, it is first expressed in skeletal and nervous tissue; vitamin K is therefore almost certainly essential to the development of the skeletal and nervous systems from their very beginnings.40

Vitamin K's activity as a cofactor for the carboxylase may only be the tip of the iceberg. In osteoblasts, the cells responsible for bone growth, the greatest concentration of vitamin K2 exists in the nucleus where the genetic material is; the second greatest concentration exists in the mitochondria, the so-called "power house" of the cell; finally, only the third greatest concentration exists in the endoplasmic reticulum where the carboxylase is found.44 We do not currently have enough information to understand the role of the K vitamins in the mitochondria or the nucleus. Osteoblasts possess a nuclear receptor for vitamin K2, suggesting it has a role as a nuclear hormone. Vitamin K2 has a higher affinity than vitamin K1 both for the nuclear receptor44 and for the mitochondrial membrane.45 There is also evidence that vitamin K2 plays a role as an antioxidant within the cells that synthesize the myelin sheath, which forms the electrical insulation of nerves.46 Although it took until the 1970s to define the function of vitamin K as a cofactor for the carboxylase enzyme, the twenty-first century may well ring in a new revolution in our understanding of this amazing vitamin with the recognition that it is, to modify a phrase coined by Tufts University's Dr. Sarah Booth, "not just for the carboxylase anymore."


Vitamin K2 and the Brain: A Closer Look

The concentration of vitamin K2 is higher in myelinated regions than in non-myelinated regions of the brain (myelin is the sheath that forms the electrical insulation of neurons) and it is correlated with the presence of important lipids such as sphingomyelin and sulfatides. The small amount of K1, by contrast, is distributed more randomly,73 suggesting that it may not be as functionally important. These lipids are part of a broader class of compounds called sphingolipids that play essential roles in the brain as structural constituents of membranes, signaling factors, and promoters of cell survival. Vitamin K2 supports the activity of the enzyme that catalyzes the initial reaction for the production of all sphingolipids as well as the enzyme that catalyzes the final step in the synthesis of sulfatides. Warfarin or dietary vitamin K deficiency cause marked decreases in the activities of these enzymes and of the levels of sulfatides in the brains of rats and mice, while the administration of either vitamin K1 or K2 restores them.46

In addition to the production of sulfatides and other sphingolipids, vitamin K2 plays at least two other important roles in the brain. The vitamin K-dependent protein gas6 promotes the survival of brain cells,74 and K vitamins, by an unknown mechanism, completely protect against the free radical-mediated death of the cells that synthesize myelin. Both an excess of glutamate and a deficiency of cystine can cause this type of cell death. Although K1 and K2 protect against glutamate toxicity equally, K2 is fifteen times more effective than K1 at counteracting the harmful effects of cystine depletion. Oxidative stress in the vulnerable infant brain can cause mental retardation, seizures, and cerebral palsy. Adequate intake of vitamin K2 during infancy may therefore protect against these diseases.75


Bacterial Production of Vitamin K2

"Vitamin K2" actually refers to a group of compounds called menaquinones. While vitamins K1 and K2 have different types of side chains, the side chains of the various menaquinones within the K2 group are all of the same type but are of varying lengths. Each of these forms is abbreviated MK-n, where "n" is a number that denotes the length of the side chain. Animal tissues exclusively synthesize MK-4, but many anaerobic bacteria synthesize other menaquinones, which they use for energy production much in the way that plants use vitamin K1.80 We can therefore obtain vitamin K2 by absorbing that which is produced by our intestinal flora or by eating fermented foods, in addition to eating animal foods which contain vitamin K2 synthesized from vitamin K1 found in grass.

Lactic acid bacteria mostly produce MK-7 through MK-10,18 while MK-10 and MK-11 accumulate in the human liver over time, presumably originating from bacterial production in the gut.81 It was once thought that intestinal bacteria were a major contributor to vitamin K status: the menaquinone content of stools is high, antibiotics have been associated with defects in blood clotting that resolve with vitamin K supplementation, and autopsies show that the great majority of vitamin K in the liver is present as "higher" menaquinones of bacterial origin. The balance of the evidence, however, challenges this view. Most of the menaquinones produced in the intestine are embedded within bacterial membranes and unavailable for absorption. Antibiotics produce vitamin K-responsive clotting defects not by reducing the intestinal production of K vitamins, but by inhibiting the enzyme within the human body that recycles them. Finally, the liver appears to accumulate higher menaquinones not because it is supplied with them abundantly but because it does not use them efficiently. Intestinal production of menaquinones therefore likely makes some contribution to vitamin K status, but one that is very small.80

Fermented foods such as sauerkraut, cheese, and natto, a soy dish popular in Eastern Japan, contain substantial amounts of vitamin K2. Natto, in fact, contains the highest amount of any food measured; nearly all of it is present as MK-7.26 MK-7 is highly effective: one recent study showed that it increased the percentage of activated osteocalcin in humans three times more powerfully than did vitamin K1.32 There are no studies available, however, comparing the efficacy of MK-7 to that of the MK-4 found in animal products. MK-9, and presumably MK-7, stays in the blood for a longer period of time than does MK-4, but this appears to be because tissues take up MK-4 much more rapidly.30 Whether the rapid uptake of MK-4 or the longer time spent in the blood by bacterial menaquinones have particular benefits or drawbacks is unclear. Future research will have to clarify whether the vitamin K2 synthesized by animal tissues and by bacteria are interchangeable, whether one is superior to the other, or whether each presents its own unique value to our health.


Supplementing with Vitamin K2

The best sources of vitamin K2 are fermented foods and grass-fed animal fats. These foods contain a wide array of nutrients that may act synergistically with vitamin K2 in ways we do not yet understand. Price 's vitamin-rich butter and butter oil concentrate provided not only vitamin K2 but also vitamin E, vitamin A, vitamin D, conjugated linoleic acid (CLA) and other nutrients. Nevertheless, some people may wish to supplement with vitamin K2 if they do not have access to high-quality food, wish to use a higher dose to treat a health condition, or want extra insurance.

Two forms of vitamin K2 supplements are commercially available: menaquinone-4 (MK-4), also called menatetrenone, and menaquinone-7 (MK-7). MK-4 is a synthetic product that is believed to be chemically and physiologically identical to the vitamin K2 found in animal fats. This form has been used in most of the animal experiments and in the Japanese osteoporosis studies. Although synthetic, it is effective, and there is no known toxicity. MK-7 is a natural extract of natto, a fermented soy food popular in Eastern Japan. MK-4 is much less expensive than MK-7, but no studies have yet compared the efficacy of these two forms.

Menaquinone-4 Supplements: Thorne Research and Carlson Laboratories both offer cost-effective MK-4 supplements. Thorne's product is a liquid supplement. The MK-4 is dissolved in a medium-chain triglyceride base (the fats found in coconut oil) with mixed tocopherols (vitamin E). Carlson's product is less expensive than Thorne's, but comes in dry capsules primarily composed of cellulose and other fillers, and allows the user less control over the dose.

Menaquinone-7 Supplements: Jarrow Formulas and Source Naturals both offer cost-effective MK-7 supplements. Source Naturals' product is less expensive, but Jarrow's contains fewer additives and certifies that the soy used to make the product is not genetically modified. Vitamin K2 supplements interfere with the activity of oral anticoagulants such as warfarin. Patients who are using warfarin should only use vitamin K2 supplements with the knowledge of the prescribing physician.


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Follow Up Questions and Answers

Question: How much K2 is recommended for adults and for children?

Answer: Unfortunately we do not have any solid numbers on the optimal or minimum intake of K vitamins, neither from modern scientific analysis nor from what people consumed in optimal traditional diets. However, we do know that virtually all adults have some degree of deficiency, whether this is very small or very substantial, and a recent study suggests that children are much more likely to be deficient, so children may actually need more because they are growing. The best thing to do would be to eat from the foods richest in vitamin K2: natto, cheese or goose liver, at least once a week and to eat from the other relatively rich foods—grass-fed butter, animal fats and fermented foods—on a daily basis. If you choose to take any of the supplements listed in the article in addition to this, there is currently no reason to believe that it is necessary to take more than the minimum dose (one drop of Thorne or one capsule of Jarrow). In the years ahead, we should see much more definitive information coming out on this, and hopefully we will also see some research reopen the questions that Price had raised about the agricultural practices that lead to the highest levels of Activator X in foods.

Question: What is the appropriate dose of vitamin K2 for maintenance and therapeutic purposes?

Answer: We do not have adequate information on dosage requirements for vitamin K2. I would think that for maintenance one should shoot for 100 mcg minimum, possibly more for children, but we will have to wait for further research to quantify this. There are unpublished anecdotes according to which some people have found up to 5-10 milligrams useful for treating specific conditions, such as autism or spider veins. At present, this is a matter that the individual must settle through experimentation.

Question: Do we know the levels of vitamin K2 in the primitive societies studied by Dr. Price?

Answer: We have no quantitative information on K2 as K2 or as Activator X in primitive societies or in Price's practice because Price did not have a means of quantifying the levels by mass—for example, how many micrograms were contained in a given food sample. Price could only compare different foods based on the intensity of the blue color yielded by the test, which he then compared to standards made from numerous different dilutions of a blue dye. So Price could say that one food was a richer or poorer source than another, but he could not determine the precise amount contained within the food.

Question: Is the K2 found in animal products or fermented foods affected by cooking?

Answer: I have yet to see any hard data on cooking losses, but everything I have read indicates that vitamin K is very heat-stable (though it can apparently incur losses from exposure to light).

Question: Is the Wulzen anti-arthritis factor in butter a separate compound from K2?

Answer: I do not know whether the Wulzen factor is a separate compound; however, others have suggested that they are separate because Wulzen found this factor to be destroyed by pasteurization, whereas Price and maybe others found activator X to be heat-stable.

Question: Fermented foods are said to be good sources of K2 but in your article you say that most of the vitamin K2 produced by bacteria in the gut is now believed to be embedded in the bacterial membranes and unavailable for absorption. One might think therefore that the vitamin K2 produced in fermented foods is similarly unavailable. Are the bacteria in fermented foods different? Does stomach digestion free up the K2?

Answer: Whether it is because some bacteria secrete the K2 or because the acidic digestion of the stomach ruptures the membranes I do not know, but the absorption of K2 from natto is near complete.

Question: Does yogurt contain vitamin K2?

Answer: Yogurt has roughly the same amount of K2 as milk, with just a tiny bit produced. This is probably because commercial yogurt is only fermented for four hours, whereas hard cheese is fermented for at least two months.

Question: Can you calculate how much K2 is in commercial versus grass-fed butter?

Answer: I do not think this can be calculated. The primary confounder is that commercial butter comes from cows in confinement operations fed massive amounts of menadione, a portion of which can be converted into K2. We have no idea at what rate this is turned into K2 and how it compares to K1 from grass as a precursor to K2, so we have no baseline from which to calculate.

Question: I'd like to use natto as a source of K2, but I have an allergy to yeast. Do you know which microorganisms ferment soy to create natto, and whether yeast is used in other parts of the process?

Answer: Natto is fermented with Bacillus subtilus, subspecies natto. Yeast is not essential to the process as far as I know, but I do not know whether the cultures tend to pick up yeast or whether for some reason some products may also deliberately use yeast. You may want to inquire with a specific manufacturer or from whomever you buy the culture if you choose to make your own.

Editor's note: Natto is definitely an acquired taste, one usually not acceptable to westerners.

 

This article appeared in Wise Traditions in Food, Farming and the Healing Arts, the quarterly magazine of the Weston A. Price Foundation, Spring 2007.

About the Author

[authorbio:masterjohn-chris]

 

 

Comments (32)Add Comment
Is there any vitamin K2 in butter?
written by Michal Mitrega, Apr 05 2014
Please referencee me to some article that states actually how much Vitamin K2 is in butter from grass-fed cows. On both FoodComp and WholeFoodsCatalog, the content of Vitamin K in butter is zero.

Don't get me wrong, butter really works for dental health but I don't really think that K2 is responsible. I simply couldn't find butter composition on the internet that measures any K2 content.
mK4 VS mk7
written by Kris Johnson, Mar 09 2014
Dr Kate's book is most interesting
K4 vs K7
written by Kris Johnson, Mar 09 2014
According to Kate Rheaume-Bleue in 'VitaminK2 and the Calcium Paradox' MK4 has a shorter half life in the body, so you need to take it 3 times per day, whereas MK7 lasts for many hours, so once a day is fine. MK7 recommended dose is also much smaller, than MK4, for some reason - 120 micrograms, vs 4,500 micrograms for MK4
MK4 or MK7?
written by Jon Nelson, Mar 04 2014
You can find people who advocate for MK7 and others for MK4. We're lacking comparative studies, so we just have to go with what (little) we know now. I've seen claims that MK7 is better because it raises serum levels quicker and for longer and with much lower dosage. I've seen counter claims that maybe the MK4 is more quickly going to work in tissues and not floating around in the blood and that it might be quickly activating proteins and that effect lasts a while even if it doesn't show up in the blood. I've seen claims that MK4 was studied in Japan for osteoporosis and therefore we can't say that MK7 would have the same effect, but this ignores the fact that bone fractures in Japan are negatively correlated with natto consumption (MK7). I've seen claims that some MK7 is converted to MK4 in the body. Until the studies are done, I think we need to conclude that we don't get enough K2 and we need to get more and they are both very good, perhaps in some different but also overlapping ways. My solution is to eat my home made natto with most meals and to occasionally supplement with Super K from Life Extension. It has MK4, MK7 and K1.
MK 4 or MK7?
written by Jen McCarthy, Feb 17 2014
Thank you for this wonderful article. I'm still confused though as to whether we should be trying to obtain MK 4 Vitamin K2, or will any K2 be as potent? If anyone could clear up that confusion, I'd greatly appreciate it! smilies/smiley.gif
Benefit could also be bacteria/enzymes
written by Jakob, Oct 01 2013
I think it may very well turn out that the benefits of cheese and natto is not so much the MK-7 as a variety of other components produced by the bacteria, like enzymes, or possibly their ability to adjust intestinal flora to a more desirable one (atherosclerosis may also possibly be caused by bacteria and improperly digested foods that enters the body as a result of leaky gut and malabsorption).

It is not clear how effective isolate MK-7 is, one study from Norway showed it had no effect (200 mcg/day) on osteoporosis after 1 year of supplementation.

I just saw this study suggesting Kefir could be beneficial for atherosclerosis: http://www.athero.org/commentaries/comm1086.asp But there´s not much K2 in kefir.

Over the years a variety of components (not the K2) has been identified in natto to allegedly reduce atherosclerosis, one of those is sold as the supplement nattokinase (an enzyme that is supposed to break down plaque in arteries).

In the end we may not really need any supplements, except possibly vitamin D3 for people living far north that doesn´t eat their traditional high marine food diet.

Most nutrients are simply available in a good nourishing traditional diet. But I think a focus on the digestibility of foods is essential. This applies to enzymes, so for example eating soft boiled eggs will preserve a ton of enzymes in the yolk, raw honey can provide huge quantities of amylase to break down starches, and fermented foods like kefir will also already have produced various enzymes, ditto for sauerkraut and other fermented foods. Sourdough bread is also easy to digest.

Fats, sugar, starches may cause problems with inadequate lecithin/choline and acids. So one would want to eat avocado as guacemole (with lemon juice/vinegar), olive oil dressing mixed with vinegar. Etc.
...
written by Jon Nelson, Sep 28 2013
Natto is a wonderful fermented food. There are hundreds of years of history of its successful use in Japan. It is easy and inexpensive to make, but the big hurdle it has outside Japan is it's taste and to a lesser extent its texture. Traditional Japanese taste has preferred strong smelling and tasting natto. More recently their preference has been shifting to more mild tasting natto. The ammonia smell is the result of which strain of natto bacteria is used and of partial protein breakdown and probably is not important for health benefits. When you make your own natto you can chose organic soy beans or many other beans. While soy beans are ideal, there are ways to feed the natto a little something extra. I have mixed biotin and sugar with the inoculant with good results. I mostly use navy beans, but also like to make a garbanzo natto humus that most people find as good as any humus. I moderate a Yahoo Group on natto that serves as a repository for information on how to make natto, alternative beans to use, recipes, how to get used to the taste and a place to ask questions. Unfortunately Yahoo has recently changed their format, making it harder to use. The URL for the group is:
groups.yahoo.com/neo/groups/nattosupport/info (I've left the http// off the front)
If you are not familiar with the new Yahoo Neo format, you will need to click on 'Conversations' to see the messages.

My belief is that most of us should be eating natto and I've found the more I eat the more I like it.

/Jon
...
written by jack miller, Sep 11 2013
I just added some natto to my chilli and its really not so bad. It could easily go into winter soup or stew.
natto flavour
written by jack miller, Sep 11 2013
I just made some chilli and added some natto for the first time. It barely made a dent in the flavour so I added a bunch more. It adds an earthy flavour and is not horrible.

From now on I'll simply put a dash in my soup or stew and maybe even screen out the soy beans.
Mr.
written by Heinz Pflieger, Jun 18 2013
As a WAPF member I read as much as I possibly can. Well aware of the good stuff written by Chris Masterjohn. Although I have embarked on a daily exercise program and nutritional program and have my Osteoarthritis pretty well under control it seems, after my 5 year daily effort - I would love to know: Once calcium deposits have developed around the bone openings in the spine and other soft tissue areas; does the right nutrition and K2 lead eventually to a reduction of these calcium deposits?
Thanks kindly, Heinz.
Why Goose Liver?
written by Jeremy Burchett, May 11 2013
Why is goose liver so high in K2? And is beef liver not high enough in K2 to make the list? I ask because goose liver is hard to find (for most people) but grass fed beef liver and pastured chicken liver is not. I guess I'll have to find goose liver. Oh and People!... Eat your Natto! for the benefit of your body!... yes it tastes like socks but you get used to it and soon will crave it. Its my first day on it and I instantly feel an effect, just like my first day on Cod liver oil and high vit. butter oil.
Let's find those sacred foods and eat them!
Some more confusion about K2...
written by Theonat, Mar 17 2013
I am currently reading the 2012 book "Vitamin K2 and the Calcium Paradox." In it the author references this very article a couple times. However, she claims certain things about Vitamin K2 which seems wrong or contradictive.

1) She claims neither K1 nor K2 can be stored. Is that true? I thought they were stored in the liver, pancreas, brain, and other places?

2) She says that the enzyme used to recycle K1 does NOT recycle K2, and thus warfarin would have no effect on K2.

3) She claims that MK-7 is much more effective than MK-4 because 120mcg of MK-7 reaps the same amount of benefits as 4,500mcg of MK-4.

4) Her graphic illustration of the MK-4 and MK-7 molecules seems... odd. I can't quite put my finger on it, but I think it's a mistake. The two-ring structure is angled differently on MK-7, and she writes H3C where CH3 should be placed. It's hard to explain, but her illustration is on page 66. Can someone else verify this? Is her graphic wrong? I cannot find this graphic anywhere else on the internet.

The confusion does not stop at Vitamin K, either. Earlier in the book she admits that the lipid hypothesis (saturated fat / cholesterol) is outdated and incorrect. However, later in the book she claims pastured animal foods are healthier because they contain LESS saturated fat and cholesterol. Huh? I guess the propaganda is so strong that nutritionists don't even realize when they perpetuate it. She does this "flip flop" again in later chapters.

Are these true mistakes on her part, or has new research shown otherwise? She does cite references and doesn't pull stuff out of nowhere. She seems well verse and appears to be a good researcher. After all, she references Chris Masterjohn a couple of times.

So what I'm saying is that the more I read about nutrition (vitamin K2 in particular), the more confused I become. Can any of you, including Chris, help clear this up for me? It would mean a lot to me.
K1 conversion into K2 in fermented foods
written by David J, Feb 17 2013
Is all the vitamin K1 content in cabbage converted into vitamin K2 in sauerkraut (and the same for all other fermented foods) or is the vitamin K1 content still present in sauerkraut?
K2 and warfarin
written by Kris Johnson, Dec 14 2012
It is my understanding that it is easier to adjust warfarin dose when you are consuming a steady amount of vitamin K1 from greens, etc. Vitamin K2 has a weak effect on blood clotting. Unfortunately your doctor may not know anything about the benefits of K2, so it might help to bring in this article. You need those grass-fed animal products to get your K2.
To clot or not to clot
written by Bella, Nov 17 2012
I am taking the Blue Ice Royal butter oil with a view to keeping calcium out of my arteries, but recently had 2 long episodes of rapid atrial fibrillation, and there is warferin in my future, I can see it in my doctor's eyes! I am looking at nattokinase instead, but I want to understand about the differences between K1 and K2 as you describe them here.
K1 is involved in clotting ( I should note) and K2 is more to do with calcium. We make K2 from K1. You don't say the dynamic goes the other way, but you do say that people on warferin should not take K2, at least make doctor aware so he can put your warferin dose up, I expect! So on the one hand K2 doesn't seem to be a clotting no no, but on the other it does, Which is it please? If both, what is the mechanism? Can I keep taking the k2 even though I am now officially at risk of stroke because my heart might produce clots.

Thank you!
...
written by Polly Kraemer, May 06 2012
Have you heard of Nature's Pasture high vitamin butter oil/cod liver oil blend? That is what I take. Is this an appropriate supplement for the k1 and k2? Xfactor.

How many a day? I have many many health problems that no traditional doctor seems to know what to do.

Please help
MD
written by Stanley Lang, Apr 09 2012
Do you know if organic apple cider vinegar has K2 and if so how much?
X Factor
written by Claus, Jan 30 2012
It will be interesting to follow the X Factor this year :-)
K1 and Diabetes, Strontium & D3
written by Ted Birnbaum, Dec 02 2011
I normally take 100 micrograms of K1 every other day. I decided to stop it completely to see its effect. After 4.5 days of no K1, my sleep was interrupted about every 2 hours by the need to urinate. And each time I awoke my mouth was unusually dry (parched) and thirsty. I also had a bad (as in unable to put socks on or tie shoes without bad pain) ache in my left hip which REFUSED TO HEAL. I took many supplements which usually speed healing for me, with no effect. During the daytime, I also noticed an increased need to urinate & drink water. My weight has also been on the decline, going from 157 to 147 over the last 6 months...although there were probably additional reasons for that. Prior to this experience there was another brief interval when I had increased frequency of nightly urination....when I was taking antibiotics for my teeth. That must have lowered my good intestinal bacteria count...which produces K1. After 2 nights of high frequency urination, I took 200 micrograms of K1 and also had a serving of good yogurt. That nite I slept 5.5 hours before awakening and needing to urinate (about normal for me)...& slept well afterwards. My mouth was also not parched like the previous nites. During the daytime I also noticed significant healing of my left hip and less need to urinate or drink water. I again took 100 micrograms of K1 and didn't have to urinate excessively. K1 is much cheaper than K2 & that's why I am using it, & I was hoping my body would convert some of the K1 to K2. I am male, 63 years old with an ideal weight of 148? and usually do not suffer from an excessive need to urinate. After reading your very revealing article, I may buy some K2. I have also used strontium citrate & believe it helped my teeth...but found strontium in EXCESS could hurt my sleep, heart beat & even the teeth in extreme excess. All those areas require a good mineral balance in the blood and could be hurt by excessive movement of calcium from the blood to the teeth & bones. I wonder if K2 will exhibit the same effect. I also observed that vitamin D3 works in opposition to strontium. I've had trouble with D3..hurting my sleep..wonder if that will be cured by K2 in light of this article
...
written by Donna, Jun 24 2011
Wow!!!! this was an awesome article and so informative. I have Osteoporosis and am taking a combo of D3&K2 along with Strontium Citrate and other bone supplements. Have you heard of Strontium Citrate for bones?
...
written by Brandon Berg, May 18 2011
Other way around, Marcus. Natto is a Japanese compound word whose components are natsu (storage) and to (bean). Soybeans kept in storage for a long time, i.e., fermented. The subspecies of bacteria is named for the food in which it is found.

And I agree with Chris that it's definitely an acquired taste. I tried it once at a sushi restaurant several years ago and thought it was pretty terrible. In light of this information, I may have to head down to Chinatown and give it another try.
VitaminK2 or VitaminK4?
written by Lisa, Mar 27 2011
I have read your article and I would like to take supplements of vitamin K2 (that are mentioned in your article) as I take calcium supplements,
it seems that the one derived from Natto one is the best
However, as Dr Mercola states, all soy products, especially the fermented ones, are highly goitrogenic, and I have some hypothyroid conditions
So my question is, would that be the same if I use the Vitamin K2 ( MK4) instead than the MK7? As it seems that the MK4 is not derived from soy, do you know if it is equally efficient?
Thanks
Lisa
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written by Tony Rutz, Jan 05 2011
Do you know if K2 supplementation been shown to reverse aortic stenosis?
Warfarin and Vitamin K2
written by PhilM, Dec 16 2010
Chris,

Wow! What an information rich article this is! I am still trying to digest the details.

Does eating foods rich in K2 (natto especially) affect anti-coagulation? I am especially curious on how K2 differs from K1 in this respect.

Natto question
written by Marcus, Dec 04 2010
Judging from the last answer to the last question, am I to understand that Natto is named after the bacteria it is produced by?

Marcus
High Vitamin Butter Oil
written by jimmyv, Aug 27 2010
If High Vitamin Butter Oil is known to contain the richest source of Activator X, does this mean that it is rich in K2? In the article above, the 'comparison' reference makes it seem like the butter oil is a great substitution for K2, since it provides very similar benefits. Am I way off on this? Is K2 and Green Pastures Butter Oil one in the same? Also, if it is still undetermined how much K2 is the right amount, how do we know that taking the recommended doses of CLO combined with HVBO contains a safe and sufficient amount of D and A and K2 to provide all the benefits referenced above?

Thanks! smilies/smiley.gif
Will K2 interfere with Aspirin
written by rashmie, May 15 2010
Hello,
This is a very informative article. It can be revolutionary to know that Vitamin K1 and K2 perform specific functions. That, K1 primarily fulfills the blood clotting functionality while K2 activates the protein that allows calcium to stay where it should - in the bones, rather than on soft tissues.

If K2 has this specific function of directing calcium to the bones, then can I safely supplement my mother with K2 who's recently undergone a Bental surgery (replacement of the aorta and valves) and she was on Warfarin initially for 2 months and now is removed from that but is put on Aspirin? My mother has severe bone problems, has Osteopenia and is really deficient in D3.

Your input will be much appreciated. Mym mom is really suffering with all sorts of pain all over her body.

Thanks much.
Vit. K2
written by Colleen Mulvihill, May 10 2010
I have been having a hard time digesting food, problems thinking,nerve problems, heart palpatations, breathing problems. I know I am low in Vit K2 per homopathic. Could this be the cause. I was on Warafarin for about 1 year because I had a blood clot in my leg. Since I have taken Warfarin, I have had so may symptoms...extreme tiredness, confusion and so much more. What would you recommend? I never feel well. Is there a standard blood test for Vit. K2

Thank you,

Colleen
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written by Tim Zuffi, Apr 13 2010
I have read the scientific papers showing the connections of vitamin K2 insufficiency/deficiency to osteoporosis, calcium deposits in the arteries and cartilage etc. I found this paper (http://bloodjournal.hematology...type=HWCIT) particularly impressive because it shows how increasing the consumption of vitamin K2 seems to reverse arterial calcification. What I would like to know is if anyone is doing a similar study with either people with osteophytes/bone spurs or with osteoarthritis, given that these diseases seem to be other manifestations of shuttling calcium to all the wrong places.
i have osteoporosis and i want to change that
written by joellenoelle, Feb 25 2010
informative article. I can't belive my dr wants me to take dangerous osteoporosis drugs when all i really may need is some healthy eating habits and some vitK2
a must read for usre
Kidney Stones and K2
written by Don, Feb 16 2010
I have suffered from kidney stones that I am unable to pass without surgical intervention. To think that it might be a lack of vitamin K2 all along is encouraging. There are so many theories about the cause of Kidney Stones including too much calcium, oxalate,cadmium, fluoride... it has my head spinning. I always found the idea of avoiding the list of 200 oxalate containing foods recommended by my local hospital's website could do more harm than good as many are nutritious.

Anyone suffering from a Calcium/oxalate kidney stone please try drinking the undiluted juice of several lemons and/or Apple Cider Vinegar mixed with a small amount of water. I had 2 Kidney stones that disappeared minutes after I drank these substances. Please don't believe me, try it yourself!

Great Article!
written by Dr. Alicia Armitstead, Feb 14 2010
I found this article full of great info that was well referenced. A must read for anyone interested in nutrition!

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Last Updated on Wednesday, 05 June 2013 16:02